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    ChromaDex rebaudioside a standards
    Rebaudioside A Standards, supplied by ChromaDex, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rebaudioside+a/Rebaudioside+A/10__3390_slash_agriculture16020159-106-6-9
    Average 94 stars, based on 9 article reviews
    rebaudioside a standards - by Bioz Stars, 2026-09
    94/100 stars

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    Expressing:

    Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods
    Article Snippet: The following reaction conditions were used: 50 mM potassium phosphate buffer, pH 7, 3 mM MgCl2, 50° C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 4 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3.C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 4 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3. ... The top 84 variants were retested with the same conditions using 1 mM stevioside (Chromadex, >94% purity), 1 mM rebaudioside A (>97% purity), or rebaudioside D (Chromadex, >93% purity).. The resulting engineered GT variant polypeptides are listed in Table 11.1.The resulting engineered GT variant polypeptides are listed in Table 11.1.

    Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods
    Article Snippet: The following reaction conditions were used: 50 mM potassium phosphate buffer, pH 7, 3 mM MgCl2, 50° C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 4 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3.C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 4 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3. ... The top 84 variants were retested with the same conditions using 1 mM stevioside (Chromadex, >94% purity), 1 mM rebaudioside A (>97% purity), or rebaudioside D (Chromadex, >93% purity).. The resulting engineered GT variant polypeptides are listed in Table 11.1.The resulting engineered GT variant polypeptides are listed in Table 11.1.

    Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods
    Article Snippet: The following reaction conditions were used: 50 mM potassium phosphate buffer, pH 7, 3 mM MgCl2, 50° C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 1 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3.C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 1 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3. ... The top 84 variants were retested with the same conditions using 1 mM stevioside (Chromadex, >94% purity), 1 mM rebaudioside A (>97% purity), or rebaudioside D (Chromadex, >93% purity).. The resulting engineered GT variant polypeptides are listed in Table 12.1.The resulting engineered GT variant polypeptides are listed in Table 12.1.

    Article Title: Effects of Seed Processing with Cold Plasma on Growth and Biochemical Traits of Stevia rebaudiana Bertoni Under Different Cultivation Conditions: In Soil Versus Aeroponics.
    Article Snippet: The standards of rutin, gallic acid, Folin–Ciocalteu phenol reagent, HPLC-grade methanol, 2,2-diphenyl-1-picrylhydrazyl, and ethanol were obtained from Sigma Aldrich (St. Louis, MO, USA).The standards of rutin, gallic acid, Folin–Ciocalteu phenol reagent, HPLC-grade methanol, 2,2-diphenyl-1-picrylhydrazyl, and ethanol were obtained from Sigma Aldrich (St. Louis, MO, USA).. Stevioside and rebaudioside A were purchased from ChromaDex (Los Angeles, CA, USA).. HPLC-grade acetonitrile and sodium acetate were obtained from Sharlau Chemie S. A. (Sentmenat, Spain).HPLC-grade acetonitrile and sodium acetate were obtained from Sharlau Chemie S. A. (Sentmenat, Spain).

    Article Title: Effects of Seed Processing with Cold Plasma on Growth and Biochemical Traits of Stevia rebaudiana Bertoni Under Different Cultivation Conditions: In Soil Versus Aeroponics
    Article Snippet: The standards of rutin, gallic acid, Folin–Ciocalteu phenol reagent, HPLC-grade methanol, 2,2-diphenyl-1-picrylhydrazyl, and ethanol were obtained from Sigma Aldrich (St. Louis, MO, USA).The standards of rutin, gallic acid, Folin–Ciocalteu phenol reagent, HPLC-grade methanol, 2,2-diphenyl-1-picrylhydrazyl, and ethanol were obtained from Sigma Aldrich (St. Louis, MO, USA).. Stevioside and rebaudioside A were purchased from ChromaDex (Los Angeles, CA, USA).. HPLC-grade acetonitrile and sodium acetate were obtained from Sharlau Chemie S. A. (Sentmenat, Spain).HPLC-grade acetonitrile and sodium acetate were obtained from Sharlau Chemie S. A. (Sentmenat, Spain).

    Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods
    Article Snippet: The following reaction conditions were used: 50 mM potassium phosphate buffer, pH 7, 3 mM MgCl2, 50° C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 1 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3.C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 1 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3. ... The top 84 variants were retested with the same conditions using 1 mM stevioside (Chromadex, >94% purity), 1 mM rebaudioside A (>97% purity), or rebaudioside D (Chromadex, >93% purity).. The resulting engineered GT variant polypeptides are listed in Table 12.1.The resulting engineered GT variant polypeptides are listed in Table 12.1.

    Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods
    Article Snippet: The following reaction conditions were used: 50 mM potassium phosphate buffer, pH 7, 3 mM MgCl2, 50° C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 4 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3.C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 4 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3. ... The top 84 variants were retested with the same conditions using 1 mM stevioside (Chromadex, >94% purity), 1 mM rebaudioside A (>97% purity), or rebaudioside D (Chromadex, >93% purity).. The resulting engineered GT variant polypeptides are listed in Table 11.1.The resulting engineered GT variant polypeptides are listed in Table 11.1.

    High Performance Liquid Chromatography:

    Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods
    Article Snippet: The following reaction conditions were used: 50 mM potassium phosphate buffer, pH 7, 3 mM MgCl2, 50° C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 4 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3.C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 4 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3. ... The top 84 variants were retested with the same conditions using 1 mM stevioside (Chromadex, >94% purity), 1 mM rebaudioside A (>97% purity), or rebaudioside D (Chromadex, >93% purity).. The resulting engineered GT variant polypeptides are listed in Table 11.1.The resulting engineered GT variant polypeptides are listed in Table 11.1.

    Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods
    Article Snippet: The following reaction conditions were used: 50 mM potassium phosphate buffer, pH 7, 3 mM MgCl2, 50° C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 4 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3.C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 4 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3. ... The top 84 variants were retested with the same conditions using 1 mM stevioside (Chromadex, >94% purity), 1 mM rebaudioside A (>97% purity), or rebaudioside D (Chromadex, >93% purity).. The resulting engineered GT variant polypeptides are listed in Table 11.1.The resulting engineered GT variant polypeptides are listed in Table 11.1.

    Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods
    Article Snippet: The following reaction conditions were used: 50 mM potassium phosphate buffer, pH 7, 3 mM MgCl2, 50° C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 1 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3.C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 1 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3. ... The top 84 variants were retested with the same conditions using 1 mM stevioside (Chromadex, >94% purity), 1 mM rebaudioside A (>97% purity), or rebaudioside D (Chromadex, >93% purity).. The resulting engineered GT variant polypeptides are listed in Table 12.1.The resulting engineered GT variant polypeptides are listed in Table 12.1.

    Article Title: Effects of Seed Processing with Cold Plasma on Growth and Biochemical Traits of Stevia rebaudiana Bertoni Under Different Cultivation Conditions: In Soil Versus Aeroponics.
    Article Snippet: The standards of rutin, gallic acid, Folin–Ciocalteu phenol reagent, HPLC-grade methanol, 2,2-diphenyl-1-picrylhydrazyl, and ethanol were obtained from Sigma Aldrich (St. Louis, MO, USA).The standards of rutin, gallic acid, Folin–Ciocalteu phenol reagent, HPLC-grade methanol, 2,2-diphenyl-1-picrylhydrazyl, and ethanol were obtained from Sigma Aldrich (St. Louis, MO, USA).. Stevioside and rebaudioside A were purchased from ChromaDex (Los Angeles, CA, USA).. HPLC-grade acetonitrile and sodium acetate were obtained from Sharlau Chemie S. A. (Sentmenat, Spain).HPLC-grade acetonitrile and sodium acetate were obtained from Sharlau Chemie S. A. (Sentmenat, Spain).

    Article Title: Effects of Seed Processing with Cold Plasma on Growth and Biochemical Traits of Stevia rebaudiana Bertoni Under Different Cultivation Conditions: In Soil Versus Aeroponics
    Article Snippet: The standards of rutin, gallic acid, Folin–Ciocalteu phenol reagent, HPLC-grade methanol, 2,2-diphenyl-1-picrylhydrazyl, and ethanol were obtained from Sigma Aldrich (St. Louis, MO, USA).The standards of rutin, gallic acid, Folin–Ciocalteu phenol reagent, HPLC-grade methanol, 2,2-diphenyl-1-picrylhydrazyl, and ethanol were obtained from Sigma Aldrich (St. Louis, MO, USA).. Stevioside and rebaudioside A were purchased from ChromaDex (Los Angeles, CA, USA).. HPLC-grade acetonitrile and sodium acetate were obtained from Sharlau Chemie S. A. (Sentmenat, Spain).HPLC-grade acetonitrile and sodium acetate were obtained from Sharlau Chemie S. A. (Sentmenat, Spain).

    Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods
    Article Snippet: The following reaction conditions were used: 50 mM potassium phosphate buffer, pH 7, 3 mM MgCl2, 50° C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 1 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3.C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 1 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3. ... The top 84 variants were retested with the same conditions using 1 mM stevioside (Chromadex, >94% purity), 1 mM rebaudioside A (>97% purity), or rebaudioside D (Chromadex, >93% purity).. The resulting engineered GT variant polypeptides are listed in Table 12.1.The resulting engineered GT variant polypeptides are listed in Table 12.1.

    Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods
    Article Snippet: The following reaction conditions were used: 50 mM potassium phosphate buffer, pH 7, 3 mM MgCl2, 50° C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 4 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3.C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 4 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3. ... The top 84 variants were retested with the same conditions using 1 mM stevioside (Chromadex, >94% purity), 1 mM rebaudioside A (>97% purity), or rebaudioside D (Chromadex, >93% purity).. The resulting engineered GT variant polypeptides are listed in Table 11.1.The resulting engineered GT variant polypeptides are listed in Table 11.1.

    Injection:

    Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods
    Article Snippet: The following reaction conditions were used: 50 mM potassium phosphate buffer, pH 7, 3 mM MgCl2, 50° C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 4 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3.C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 4 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3. ... The top 84 variants were retested with the same conditions using 1 mM stevioside (Chromadex, >94% purity), 1 mM rebaudioside A (>97% purity), or rebaudioside D (Chromadex, >93% purity).. The resulting engineered GT variant polypeptides are listed in Table 11.1.The resulting engineered GT variant polypeptides are listed in Table 11.1.

    Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods
    Article Snippet: The following reaction conditions were used: 50 mM potassium phosphate buffer, pH 7, 3 mM MgCl2, 50° C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 4 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3.C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 4 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3. ... The top 84 variants were retested with the same conditions using 1 mM stevioside (Chromadex, >94% purity), 1 mM rebaudioside A (>97% purity), or rebaudioside D (Chromadex, >93% purity).. The resulting engineered GT variant polypeptides are listed in Table 11.1.The resulting engineered GT variant polypeptides are listed in Table 11.1.

    Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods
    Article Snippet: The following reaction conditions were used: 50 mM potassium phosphate buffer, pH 7, 3 mM MgCl2, 50° C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 1 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3.C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 1 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3. ... The top 84 variants were retested with the same conditions using 1 mM stevioside (Chromadex, >94% purity), 1 mM rebaudioside A (>97% purity), or rebaudioside D (Chromadex, >93% purity).. The resulting engineered GT variant polypeptides are listed in Table 12.1.The resulting engineered GT variant polypeptides are listed in Table 12.1.

    Article Title: Effects of Seed Processing with Cold Plasma on Growth and Biochemical Traits of Stevia rebaudiana Bertoni Under Different Cultivation Conditions: In Soil Versus Aeroponics.
    Article Snippet: The standards of rutin, gallic acid, Folin–Ciocalteu phenol reagent, HPLC-grade methanol, 2,2-diphenyl-1-picrylhydrazyl, and ethanol were obtained from Sigma Aldrich (St. Louis, MO, USA).The standards of rutin, gallic acid, Folin–Ciocalteu phenol reagent, HPLC-grade methanol, 2,2-diphenyl-1-picrylhydrazyl, and ethanol were obtained from Sigma Aldrich (St. Louis, MO, USA).. Stevioside and rebaudioside A were purchased from ChromaDex (Los Angeles, CA, USA).. HPLC-grade acetonitrile and sodium acetate were obtained from Sharlau Chemie S. A. (Sentmenat, Spain).HPLC-grade acetonitrile and sodium acetate were obtained from Sharlau Chemie S. A. (Sentmenat, Spain).

    Article Title: Effects of Seed Processing with Cold Plasma on Growth and Biochemical Traits of Stevia rebaudiana Bertoni Under Different Cultivation Conditions: In Soil Versus Aeroponics
    Article Snippet: The standards of rutin, gallic acid, Folin–Ciocalteu phenol reagent, HPLC-grade methanol, 2,2-diphenyl-1-picrylhydrazyl, and ethanol were obtained from Sigma Aldrich (St. Louis, MO, USA).The standards of rutin, gallic acid, Folin–Ciocalteu phenol reagent, HPLC-grade methanol, 2,2-diphenyl-1-picrylhydrazyl, and ethanol were obtained from Sigma Aldrich (St. Louis, MO, USA).. Stevioside and rebaudioside A were purchased from ChromaDex (Los Angeles, CA, USA).. HPLC-grade acetonitrile and sodium acetate were obtained from Sharlau Chemie S. A. (Sentmenat, Spain).HPLC-grade acetonitrile and sodium acetate were obtained from Sharlau Chemie S. A. (Sentmenat, Spain).

    Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods
    Article Snippet: The following reaction conditions were used: 50 mM potassium phosphate buffer, pH 7, 3 mM MgCl2, 50° C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 1 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3.C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 1 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3. ... The top 84 variants were retested with the same conditions using 1 mM stevioside (Chromadex, >94% purity), 1 mM rebaudioside A (>97% purity), or rebaudioside D (Chromadex, >93% purity).. The resulting engineered GT variant polypeptides are listed in Table 12.1.The resulting engineered GT variant polypeptides are listed in Table 12.1.

    Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods
    Article Snippet: The following reaction conditions were used: 50 mM potassium phosphate buffer, pH 7, 3 mM MgCl2, 50° C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 4 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3.C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 4 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3. ... The top 84 variants were retested with the same conditions using 1 mM stevioside (Chromadex, >94% purity), 1 mM rebaudioside A (>97% purity), or rebaudioside D (Chromadex, >93% purity).. The resulting engineered GT variant polypeptides are listed in Table 11.1.The resulting engineered GT variant polypeptides are listed in Table 11.1.

    Mutagenesis:

    Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods
    Article Snippet: The following reaction conditions were used: 50 mM potassium phosphate buffer, pH 7, 3 mM MgCl2, 50° C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 4 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3.C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 4 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3. ... The top 84 variants were retested with the same conditions using 1 mM stevioside (Chromadex, >94% purity), 1 mM rebaudioside A (>97% purity), or rebaudioside D (Chromadex, >93% purity).. The resulting engineered GT variant polypeptides are listed in Table 11.1.The resulting engineered GT variant polypeptides are listed in Table 11.1.

    Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods
    Article Snippet: The following reaction conditions were used: 50 mM potassium phosphate buffer, pH 7, 3 mM MgCl2, 50° C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 4 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3.C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 4 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3. ... The top 84 variants were retested with the same conditions using 1 mM stevioside (Chromadex, >94% purity), 1 mM rebaudioside A (>97% purity), or rebaudioside D (Chromadex, >93% purity).. The resulting engineered GT variant polypeptides are listed in Table 11.1.The resulting engineered GT variant polypeptides are listed in Table 11.1.

    Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods
    Article Snippet: The following reaction conditions were used: 50 mM potassium phosphate buffer, pH 7, 3 mM MgCl2, 50° C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 1 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3.C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 1 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3. ... The top 84 variants were retested with the same conditions using 1 mM stevioside (Chromadex, >94% purity), 1 mM rebaudioside A (>97% purity), or rebaudioside D (Chromadex, >93% purity).. The resulting engineered GT variant polypeptides are listed in Table 12.1.The resulting engineered GT variant polypeptides are listed in Table 12.1.

    Article Title: Effects of Seed Processing with Cold Plasma on Growth and Biochemical Traits of Stevia rebaudiana Bertoni Under Different Cultivation Conditions: In Soil Versus Aeroponics.
    Article Snippet: The standards of rutin, gallic acid, Folin–Ciocalteu phenol reagent, HPLC-grade methanol, 2,2-diphenyl-1-picrylhydrazyl, and ethanol were obtained from Sigma Aldrich (St. Louis, MO, USA).The standards of rutin, gallic acid, Folin–Ciocalteu phenol reagent, HPLC-grade methanol, 2,2-diphenyl-1-picrylhydrazyl, and ethanol were obtained from Sigma Aldrich (St. Louis, MO, USA).. Stevioside and rebaudioside A were purchased from ChromaDex (Los Angeles, CA, USA).. HPLC-grade acetonitrile and sodium acetate were obtained from Sharlau Chemie S. A. (Sentmenat, Spain).HPLC-grade acetonitrile and sodium acetate were obtained from Sharlau Chemie S. A. (Sentmenat, Spain).

    Article Title: Effects of Seed Processing with Cold Plasma on Growth and Biochemical Traits of Stevia rebaudiana Bertoni Under Different Cultivation Conditions: In Soil Versus Aeroponics
    Article Snippet: The standards of rutin, gallic acid, Folin–Ciocalteu phenol reagent, HPLC-grade methanol, 2,2-diphenyl-1-picrylhydrazyl, and ethanol were obtained from Sigma Aldrich (St. Louis, MO, USA).The standards of rutin, gallic acid, Folin–Ciocalteu phenol reagent, HPLC-grade methanol, 2,2-diphenyl-1-picrylhydrazyl, and ethanol were obtained from Sigma Aldrich (St. Louis, MO, USA).. Stevioside and rebaudioside A were purchased from ChromaDex (Los Angeles, CA, USA).. HPLC-grade acetonitrile and sodium acetate were obtained from Sharlau Chemie S. A. (Sentmenat, Spain).HPLC-grade acetonitrile and sodium acetate were obtained from Sharlau Chemie S. A. (Sentmenat, Spain).

    Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods
    Article Snippet: The following reaction conditions were used: 50 mM potassium phosphate buffer, pH 7, 3 mM MgCl2, 50° C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 1 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3.C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 1 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3. ... The top 84 variants were retested with the same conditions using 1 mM stevioside (Chromadex, >94% purity), 1 mM rebaudioside A (>97% purity), or rebaudioside D (Chromadex, >93% purity).. The resulting engineered GT variant polypeptides are listed in Table 12.1.The resulting engineered GT variant polypeptides are listed in Table 12.1.

    Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods
    Article Snippet: The following reaction conditions were used: 50 mM potassium phosphate buffer, pH 7, 3 mM MgCl2, 50° C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 4 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3.C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 4 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3. ... The top 84 variants were retested with the same conditions using 1 mM stevioside (Chromadex, >94% purity), 1 mM rebaudioside A (>97% purity), or rebaudioside D (Chromadex, >93% purity).. The resulting engineered GT variant polypeptides are listed in Table 11.1.The resulting engineered GT variant polypeptides are listed in Table 11.1.

    Concentration Assay:

    Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods
    Article Snippet: The following reaction conditions were used: 50 mM potassium phosphate buffer, pH 7, 3 mM MgCl2, 50° C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 4 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3.C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 4 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3. ... The top 84 variants were retested with the same conditions using 1 mM stevioside (Chromadex, >94% purity), 1 mM rebaudioside A (>97% purity), or rebaudioside D (Chromadex, >93% purity).. The resulting engineered GT variant polypeptides are listed in Table 11.1.The resulting engineered GT variant polypeptides are listed in Table 11.1.

    Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods
    Article Snippet: The following reaction conditions were used: 50 mM potassium phosphate buffer, pH 7, 3 mM MgCl2, 50° C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 4 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3.C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 4 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3. ... The top 84 variants were retested with the same conditions using 1 mM stevioside (Chromadex, >94% purity), 1 mM rebaudioside A (>97% purity), or rebaudioside D (Chromadex, >93% purity).. The resulting engineered GT variant polypeptides are listed in Table 11.1.The resulting engineered GT variant polypeptides are listed in Table 11.1.

    Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods
    Article Snippet: The following reaction conditions were used: 50 mM potassium phosphate buffer, pH 7, 3 mM MgCl2, 50° C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 1 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3.C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 1 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3. ... The top 84 variants were retested with the same conditions using 1 mM stevioside (Chromadex, >94% purity), 1 mM rebaudioside A (>97% purity), or rebaudioside D (Chromadex, >93% purity).. The resulting engineered GT variant polypeptides are listed in Table 12.1.The resulting engineered GT variant polypeptides are listed in Table 12.1.

    Article Title: Effects of Seed Processing with Cold Plasma on Growth and Biochemical Traits of Stevia rebaudiana Bertoni Under Different Cultivation Conditions: In Soil Versus Aeroponics.
    Article Snippet: The standards of rutin, gallic acid, Folin–Ciocalteu phenol reagent, HPLC-grade methanol, 2,2-diphenyl-1-picrylhydrazyl, and ethanol were obtained from Sigma Aldrich (St. Louis, MO, USA).The standards of rutin, gallic acid, Folin–Ciocalteu phenol reagent, HPLC-grade methanol, 2,2-diphenyl-1-picrylhydrazyl, and ethanol were obtained from Sigma Aldrich (St. Louis, MO, USA).. Stevioside and rebaudioside A were purchased from ChromaDex (Los Angeles, CA, USA).. HPLC-grade acetonitrile and sodium acetate were obtained from Sharlau Chemie S. A. (Sentmenat, Spain).HPLC-grade acetonitrile and sodium acetate were obtained from Sharlau Chemie S. A. (Sentmenat, Spain).

    Article Title: Effects of Seed Processing with Cold Plasma on Growth and Biochemical Traits of Stevia rebaudiana Bertoni Under Different Cultivation Conditions: In Soil Versus Aeroponics
    Article Snippet: The standards of rutin, gallic acid, Folin–Ciocalteu phenol reagent, HPLC-grade methanol, 2,2-diphenyl-1-picrylhydrazyl, and ethanol were obtained from Sigma Aldrich (St. Louis, MO, USA).The standards of rutin, gallic acid, Folin–Ciocalteu phenol reagent, HPLC-grade methanol, 2,2-diphenyl-1-picrylhydrazyl, and ethanol were obtained from Sigma Aldrich (St. Louis, MO, USA).. Stevioside and rebaudioside A were purchased from ChromaDex (Los Angeles, CA, USA).. HPLC-grade acetonitrile and sodium acetate were obtained from Sharlau Chemie S. A. (Sentmenat, Spain).HPLC-grade acetonitrile and sodium acetate were obtained from Sharlau Chemie S. A. (Sentmenat, Spain).

    Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods
    Article Snippet: The following reaction conditions were used: 50 mM potassium phosphate buffer, pH 7, 3 mM MgCl2, 50° C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 1 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3.C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 1 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3. ... The top 84 variants were retested with the same conditions using 1 mM stevioside (Chromadex, >94% purity), 1 mM rebaudioside A (>97% purity), or rebaudioside D (Chromadex, >93% purity).. The resulting engineered GT variant polypeptides are listed in Table 12.1.The resulting engineered GT variant polypeptides are listed in Table 12.1.

    Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods
    Article Snippet: The following reaction conditions were used: 50 mM potassium phosphate buffer, pH 7, 3 mM MgCl2, 50° C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 4 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3.C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 4 h. The reactions were quenched by adding 10 μl assay 90 μL acetonitrile with 0.2% formic acid and centrifuged 10 m at 4° C. The supernatants were diluted 1:10 in water and analyzed by RapidFire SPE-MS/MS as described in Table 9.3. ... The top 84 variants were retested with the same conditions using 1 mM stevioside (Chromadex, >94% purity), 1 mM rebaudioside A (>97% purity), or rebaudioside D (Chromadex, >93% purity).. The resulting engineered GT variant polypeptides are listed in Table 11.1.The resulting engineered GT variant polypeptides are listed in Table 11.1.



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